Coding

Part:BBa_K3760000

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-08-27)


Rfx Cas13d coding sequence

Rfx Cas13d is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13d undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.The original RfxCas13d protein gene contains rare codons. We optimized the codon to make it could be expressed in common E. coli protein expression strains, such as E. coli BL21(DE3).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2377
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None